Exploring the Role of CRISPR-Cas9 in Genetic Engineering: Advancements, Applications, and Ethical Issues


Abstract views: 134 / PDF downloads: 83

Authors

  • Nursel Eski HS of Innovation – Katy, USA
  • Huda Asif HS of Innovation – Katy, USA
  • Jacqueline Crespo HS of Ingenuity – Houston, USA
  • Yakup Bayar The University of Texas at Austin, USA

DOI:

https://doi.org/10.31039/plic.2024.11.260

Keywords:

CRISPR-Cas9, gene therapy, genome engineering

Abstract

Since its discovery in 1987, the emerging genome-modification technology CRISPR-Cas9 has augmented the ever-evolving field of genetic engineering through its advancements in precision and accuracy to simplify efficient genome alteration. This paper introduces the history of CRISPR-Cas9 and explores its underlying mechanisms and advancements. Significant technological advancements have enhanced the precision and efficiency of CRISPR-Cas9 in genetic engineering. Innovations like base and prime editors minimize the unintended off-target effects, improving the accuracy of gene editing. The development of advanced delivery methods, such as magnetic nanoparticles, allows for faster delivery of editing components to their intended destination with greater precision. This complex has a wide range of applications in fields such as medicine, agriculture, and industrial biotechnology. CRISPR-Cas9 has recently grown popular among gene therapy studies for genetic disorders in addition to cancer research for further understanding of cancer cell mechanisms. In agricultural settings, this tool has been used to modify crops to withstand environmental constraints to increase crop yield and alter nutritional content. CRISPR-Cas9’s role in industrial biotechnology is also discussed as modifying the metabolic pathways of microorganisms to facilitate higher biofuel production. Ethical considerations related to the technology such as safety, possible human germline misuse, and ecological effects of GMOs have catalyzed social and political restraints with pertinent case studies. Challenges such as off-target effects, generational consequences, and unequal access are mentioned. Nevertheless, ethical questions remain without prominent responses. The future of genetic engineering is in the hands of geneticists working with CRISPR-Cas9 to offer greater treatment options for fatal genetic disorders. This review aims to provide a better understanding of CRISPR-Cas9’s significant use and role in genetic engineering.

References

Adli, M. (2018). The CRISPR tool kit for genome editing and beyond. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-04252-2

Anik, Muzahidul I., et al. “Biomedical Applications of Magnetic Nanoparticles.” Elsevier eBooks, 2021, pp. 463–97. https://doi.org/10.1016/b978-0-12-823688-8.00002-8.

Ansori, A. N., and et al (2023). Application of CRISPR-Cas9 genome editing technology in various fields: A review. Narra J, 3(2), e184–e184. https://doi.org/10.52225/narra.v3i2.184

Asmamaw, M., & Zawdie, B. (2021). Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing. Biologics : Targets & Therapy, 15(1), 353–361. https://doi.org/10.2147/BTT.S326422

Anzalone, Andrew V., et al. “Search-and-replace Genome Editing Without Double-strand Breaks or Donor DNA.” Nature, vol. 576, no. 7785, Oct. 2019, pp. 149–57. https://doi.org/10.1038/s41586-019-1711-4.

Barman, A., Deb, B., & Chakraborty, S. (2019). A Glance at Genome Editing with CRISPR–Cas9 Technology. Current Genetics, 66. https://doi.org/10.1007/s00294-019-01040-3

CBAN. (2023). Environmental Impacts. Canadian Biotechnology Action Network. https://cban.ca/gmos/issues/environmental-impacts/#:~:text=Biodiversity%20Loss%3A%20The%20use%20of

Chinese Scientist Who Gene-Edited Babies Is Back In Lab After Jail Time. (n.d.-a). NDTV.com. https://www.ndtv.com/world-news/chinese-scientist-who-gene-edited-babies-is-back-in-lab-after-jail-time-5369252#:

Cortez, C. (2015, March 12). CRISPR 101: Homology Directed Repair. Blog.addgene.org. https://blog.addgene.org/crispr-101-homology-directed-repair

Donohoue, P. D., Barrangou, R., & May, A. P. (2018). Advances in Industrial Biotechnology Using CRISPR-Cas Systems. Trends in Biotechnology, 36(2), 134–146. https://doi.org/10.1016/j.tibtech.2017.07.007

Du, Yimin, et al. “CRISPR/Cas9 Systems: Delivery Technologies and Biomedical Applications.” Asian Journal of Pharmaceutical Sciences, vol. 18, no. 6, Nov. 2023, p. 100854. https://doi.org/10.1016/j.ajps.2023.100854.

Firouzeh Morshedzadeh, et al.(2023). An Update on the Application of CRISPR Technology in Clinical Practice. Molecular Biotechnology, 66. https://doi.org/10.1007/s12033-023-00724-z

Friends of the Earth. (2018, September 12). NEW REPORT: Gene Editing in Agriculture Poses New Risks to Health, Environment. Friends of the Earth. https://foe.org/news/gene-editing-risks-health-environment/

Genetic Literacy Project. (2019b, July 23). United States: Germline / Embryonic. Global Gene Editing Regulation Tracker. https://crispr-gene-editing-regs-tracker.geneticliteracyproject.org/united-states-embryonic-germline-gene-editing/

Gostimskaya, I. (2022). CRISPR–Cas9: A History of Its Discovery and Ethical Considerations of Its Use in Genome Editing. Biochemistry (Moscow), 87(8), 777–788. https://doi.org/10.1134/s0006297922080090

Hryhorowicz, Magdalena, et al. “Improved Delivery of CRISPR/Cas9 System Using Magnetic Nanoparticles Into Porcine Fibroblast.” Molecular Biotechnology, vol. 61, no. 3, Dec. 2018, pp. 173–80. https://doi.org/10.1007/s12033-018-0145-9.

Ishino, Y., Krupovic, M., & Forterre, P. (2018). History of CRISPR-Cas from Encounter with a Mysterious Repeated Sequence to Genome Editing Technology. Journal of Bacteriology, 200(7), e00580-17. https://doi.org/10.1128/JB.00580-17

Javed, M. R., et al. (2019). Current situation of biofuel production and its enhancement by CRISPR/Cas9-mediated genome engineering of microbial cells. Microbiological Research, 219, 1–11. https://doi.org/10.1016/j.micres.2018.10.010

Kantor, Ariel, et al. “CRISPR-Cas9 DNA Base-Editing and Prime-Editing.” International Journal of Molecular Sciences, vol. 21, no. 17, Aug. 2020, p. 6240. https://doi.org/10.3390/ijms21176240.

Liu, Q., et al. (2021). Application of CRISPR/Cas9 in Crop Quality Improvement. International Journal of Molecular Sciences, 22(8), 4206. https://doi.org/10.3390/ijms22084206

McCarthy, S. (2023, July 3). Controversial Chinese scientist He Jiankui proposes new gene editing research. CNN. https://edition.cnn.com/2023/07/03/china/he-jiankui-gene-editing-proposal-china-intl

Movahedi, A., Aghaei-Dargiri, S., Li, H., Zhuge, Q., & Sun, W. (2023). CRISPR Variants for Gene Editing in Plants: Biosafety Risks and Future Directions. International Journal of Molecular Sciences, 24(22), 16241. https://doi.org/10.3390/ijms242216241

National Human Genome Research Institute. (2017, August 3). What are the Ethical Concerns of Genome Editing? National Human Genome Research Institute; National Institutes of Health. https://www.genome.gov/about-genomics/policy-issues/Genome-Editing/ethical-concr

Ng, D. (2023, November 10). A Brief History of CRISPR-Cas9 Genome-Editing Tools. Bitesize Bio. https://bitesizebio.com/47927/history-crispr/

Nutrition, C. for F. S. and A. (2020b). How GMO Crops Impact Our World. FDA. https://www.fda.gov/food/agricultural-biotechnology/how-gmo-crops-impact-our-worl

PhD, A. A. S. (2023, April 3). CRISPR-Made Greener Products and Processes in Industrial Biotechnology. GEN - Genetic Engineering and Biotechnology News. https://www.genengnews.com/resources/crispr-made-greener-products-and-processes-in-industrial-biotechnology/

Ravichandran, M., & Maddalo, D. (2023). Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modeling. Frontiers in Genetics, 14. https://doi.org/10.3389/fgene.2023.1273994

Ronald, P., & Kliegman, M. (2023). CRISPR in Agriculture. Innovative Genomics Institute (IGI). https://innovativegenomics.org/crisprpedia/crispr-in-agriculture/

Rose, B. I., & Brown, S. (2019). Genetically Modified Babies and a First Application of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR-Cas9). Obstetrics & Gynecology, 134(1), 1. https://doi.org/10.1097/aog.0000000000003327

Savulescu, J., Pugh, J., Douglas, T., & Gyngell, C. (2015c). The moral imperative to continue gene editing research on human embryos. Protein & Cell, 6(7), 476–479. https://doi.org/10.1007/s13238-015-0184-y

Schmerker, J. (2024b, April 15). CRISPR-Cas9: 10 pros and 7 cons. Integrated DNA Technologies. https://sg.idtdna.com/pages/community/blog/post/crispr-cas9-what-are-the-10-pros-and-7-cons

Schreiber, K. (2019b, December 12). India: Crops / Food. Global Gene Editing Regulation Tracker. https://crispr-gene-editing-regs-tracker.geneticliteracyproject.org/india-crops-food/

Updates on Global Regulatory Landscape for Gene-Edited Crops. (n.d.). Science Speaks. https://www.isaaa.org/blog/entry/default.asp?BlogDate=1/24/2024

Wenham, L. (2023, September 6). CRISPR in agriculture: Applications, benefits & risks. Automata. https://automata.tech/blog/crispr-agriculture/

World Health Organization. (2023). Human genome editing. World Health Organization. https://www.who.int/health-topics/human-genome-editing#tab=tab

Zhu, H., Li, C., & Gao, C. (2020). Applications of CRISPR–Cas in agriculture and plant biotechnology. Nature Reviews Molecular Cell Biology, 21(11), 661–677. https://doi.org/10.1038/s41580-020-00288-9

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Published

2024-10-12

How to Cite

Eski, N., Huda Asif, Jacqueline Crespo, & Yakup Bayar. (2024). Exploring the Role of CRISPR-Cas9 in Genetic Engineering: Advancements, Applications, and Ethical Issues. Proceedings of London International Conferences, (11), 158–170. https://doi.org/10.31039/plic.2024.11.260